Before I began running this game I made a list of breaks in the run. I hope this helps new runners who see the game as a marathon to pace yourself and plan out food/bathroom/stretching-your-legs breaks. These breaks are about 27.5 minutes in total. There are a few smaller breaks such as the 'Fuzzy pickles' photos, but these are your best chances to get up and run into the other room if needed.
Intro - 1:08 Runaway Five - 1:11 R5 Bus from Twoson - 1:04 Winters Intro - 0:32 Tessie - 1:30 Sky Runner to Threed - 2:24 Belch Door - 3:00 Bus to Desert - 0:48 Runaway Five feat. Venus - 1:04 Bus to Monkey Cave - 0:42 R5 Bus from Fourside - 1:24 Sky Runner to Winters - 1:36 Sky Runner to Summers - 1:00 Dalaam Intro - 0:38 Sailing/Kraken - 0:48 + 0:27 Submarine that happens to be yellow - 0:50 Tessie pt. 2 - 1:30 Lumine Hall Sanctuary - 1:09 Venus concert - 2:19 Magicant Intro - 1:50 Wake Up - 0:28 Robots - 0:43