Here is a brief summary of basic forms of movement and other stuff you need to know to optimize your speedruns of this game.

Movement: Running is Clyde's default and fastest form of movement when playing as him normally, jumping will slow him down.

Diving: When Clyde approaches a pit that he will have to fall through, he could fall through normally, or he could do a belly dive, which is the faster of the two. Jumping and pressing X (on Xbox) will make Clyde do the dive

Sheep: Out of all the transformations in the game, the Sheep is the most useful for Speedrunning, as Sheep Clyde runs significantly faster than his normal counterpart. The sheep is only playable in the full game.

Super Attacks: To beat a level, Clyde needs to defeat every single robot in a stage to get the exit portal to appear, hitting the robots with Clyde's normal attack will take significantly longer than using the Super Attack (which earned by collecting enough DNA strands) and Clyde can only have 3 Super Attacks available at once, but the Super Attack can immediately defeat a robot.

Mutant Chicken Attack: When Clyde is out of DNA strands to do a Super Attack, the next most optimal thing to do is attack the robot normally, and cleverly position himself so that the Mutant Chickens that appear to attack him, also attack the robot. Not to mention, does a lot more damage than Clyde's normal punches. When the Chicken is about to explode, move out of the way and go back to beating up that robot.