Found a small glitch, it's possible to "double jump" in normal form, which saves additional normal jumps. Good enough run for me, it comes down to frames now which I'm not willing to grind.

There's also a faster end strat at the frying pan, where you can land on the edge, but the death barrier is pretty precise there, so it's hard to land on there without dying, which can save several frames.

Unless someone finds another route or knows how to skip the hands I don't see this getting below 46 seconds tbh with these current strats.

]Start Time 12.867, End Time: 55.267, Frame Rate: 30, Time: 42.4[