After gameplay I retimed this run using https://somewes.com/frame-count/ with a console FPS of 59.599 which is the NeoGeo NTSC native framerate according to http://wiki.neogeodev.org/index.php?title=Framerate and raw video framerate of 30 FPS for a total of 59068 frames or a Console Time of 32:48.926. First Frame set on first dark Frame before screen opens and End Frame set on the frame of the last hit.

I use WinKawaks 1.65 with "mslug5.zip" ROM.

I time my runs manually with LiveSplit during gameplay.

I use Retroflag USB Gamepad controller and NintendoSpy as Input Display.

Thanks to FabricioJosfa!