Notable mistakes include a slightly sloppy Joka, an easily avoidable death in 6-1, falling into a teleporter unexpectedly in Ghadius, and losing a moo in the final phase of Nahatomb.
There are also known optimizations possible in 5-1, 5-2, 6-1, and 6-2 that could save an additional several seconds at least. Most of these are in Balneor's run. Neil also found some potential optimizations that are worth investigating further.
Sub-46 is probably realistically attainable with enough effort. Sub-45 seems completely out of reach for now.
It's true ! It has been done ! After more than 25 years ! A new dimension of play for this game is now upon you !
All four categories ! And submissions compatible with full-game run footage ! So you can port all of your best and proudest stage performances !
You can read about the making of start