Hello!

For Bioshock 1 - yes you must collect all plasmids. For Bioshock 2 we only need to collect the lvl 1 plasmids

For the most part, dealing with little sisters is done as little as possible, due to it taking extra time to do so. The closest category we have that fits with what you're looking for is the "All Collectibles" run. We don't end up saving every sister, but we do save most.

As for new game+, there is a category for that. Bioshock and Remastered are separate games here on speedrun.com complete with their own individual sets of leaderboards. You'll find the new game+ stuff on remastered, since the original didn't have that.

Let me know if you have any other questions, also you'll probably get better/faster results on the discord: https://discord.gg/

Hello,

There's an area in the "Guides" section that explains it all. Here's the link: https://www.speedrun.com/hl1/guide/m3lo4

TLDR - you need to zip them into a file and submit with your run.

Is there a Discord server specifically for Questathon EX?