I don't think I can take this much lower, there isn't much timesave remaining. Hit all the skips, made the 4-3 and 5-1 platform cycles, and also got a lot of good golds. Major timeloss: I lost 10 seconds on 1-5, didn't damage boost through all the ants on 2-1, took a few tries to make that jump after 2-4 skip, and played 5-5 very safe on one HP. My sum of best is currently 41:08, sub-41 is probably not happening for a long time (from me, at least).

Emulator is BizHawk 2.9 w/ mGBA core