Hi, Recently I found that you can actually defeat the sisters of fate without the amulet of the fate item, which is very important item and necessary to progress this fight. Now with this possibility we no longer need to get the item and waste time, that will save more than a minute, furthermore the fight is going to be faster by couple of seconds (-15 second If the fight went perfectly with my optimisation). This is my final optimisation:

The second sisters = Atropos

Ok I'm gonna explain what the strategy is and then what happened in the second mirror. You need strike and knock down the second sister as soon as she appears (with the hammer L1+Triangle ) not immediately though. First shot her with typhon bow and knock Lahkesis so she appears in the other mirror and prepare for the strike. It is easy to get used to the timing by practice yourself, that's pretty much it. The second mirror is very tricky the timing should be delayed a little bit ( I recommend using the spear L1+Circle ) .

About the Optimisation As you can see in the video after I destroyed the second mirror Lahkesis immediately went to the third mirror which is unusual ( and that's what you want her to do) ok now step by step. After you destroy the first mirror the second sister will go to the second mirror really fast so there's no time to knock Lahkesis and get near the mirror, but if you follow this setup you'll get it 70% of the time, and if you don't get it and she hides no worries just knock Lahkesis and she will appear. The setup is { roll > jump > L2 + Triangle with the bow and two shots } the bow is for distracted Lahkesis since we won't knock her down she will interrupt us while we're trying to strike the second sister. Lahkesis will get to the third mirror immediately due to that there will be a bug tht will break kratos out of the Quick Time Event, even with this bug this is still the best optimisation.

I hope I explained well :)