Yes
Internet speed has nothing to do with low FPS in a web game, if you have problem with low fps with your videos, it's probably because your recording software isn't optimised. Try reducing the recording quality (from 1080p to 720p for example) or lower the bitrate, it can help a lot with lags in your videos.
I had this glitch happen to me too, no idea how it happpens, but I do remember grabbing 2 flies at the same time aswell
Turbo mode is impossible, once you click on the frog to get the flies, it bugs out and you are softlocked
found 3 glitches that could be cool for the speedrun.
I saw that in the rules glitches are banned but it could be a separate category.
just a reminder that using a software to click faster is not allowed :)
This guide by wavey is very good, take a look at it https://www.speedrun.com/ting/guide/4n6tm , also practice movements and breakout and you should get a sub 4:30 pretty easily.