Retimed with Avidemux. With the slightly improved timing method, the exact time is 4:09.033.

I've used the improved routing made by @HurricaneOf87 on his 4:17 run, and added few improvements.

First, I've discovered that in the room where you need to kill all the enemies, you only need to kill the insects. You don't need to take care of the barriers. And if you are fast enough, the insects will all be in a good position to kill them quickly.

Second, I've slightly improved the way you setup the Death Warp. I've made a video you can find in the Guides section, which explain every steps.

The part which can potentially be improved is the boss fight. I feel like I got a good fight, without triggering Stasis, but I still feel like this part is my main weakness, and I won't be surprised if it can still be improved, despite the RNG factor.

The rest was basically flawless, with possible time losses only being about 0.5 second. It's the first sub 4:10, and we may need a bit more development before reaching sub 4.

Twitch VOD: