I don't use an emulator, but I do use an NES compatible machine called Retro Freak. Therefore, there are times when the menu is displayed during play.

Moderator note: Retimed to 36:54, because this leaderboard's RTA timing begins after characters have been selected for the first time. Nice run!

Start Time 12.8, End Time: 37:07.05, Frame Rate: 60, Time: 36:54.25