Can I be in a call with others, if they are saying things relating to the game at the time?

Wouldn't know bucko, I wouldn't assume that it would do anything, I've uploaded footage with YouTube playing in the Background and it got verified fine so i'm just assuming that it'd be fine but best wait for a mod to verify.

That shouldn't be a problem, it's like Twitch streamers interacting with chat during a speedrun.