Run starts at 3:20

This category requires player to complete the game with each lady once with the final time ending when the last lady gets STAGE CLEAR at the final boss.

To improve upon the current run, we discover a quicker stage 2-2 route in the hive that helps you get to Queen Bee faster. When entering the hive, players should go

LEFT>LEFT>LARVAE(RIGHT)>LEFT>RIGHT

This route is used for each girl in this run, skips several mandatory fights and allows you to reach Queen with full SP. Taking this route also save much time so you can afford to not to skip Save the Queen, something Sachiho seemingly cannot do.

For the first three girls, we also "restart" the game to skip the credits after landing the last hit on the crystal for final boss. The indicator for this is when the game ZOOMS in on the crystal and the player loses control over the character. At this point, the game is technically over so there is no reason to wait for STAGE CLEAR.

Playing on the latest version of the game 1.11 also helped a little particularly with TGO's air jumps.

I missed the restart for Mirea and lost some time, but I added splits for Crystal and Stage Clear for final time with TGO. I also miss timed a skip during Mirea segment when I tried to escape a grab and instead hit hotkey.

The order I chose is Sachiho>Usaco>Mirea>TGO. Sachiho absolutely first because she will take the longest and has difficulty skipping save the queen. Usaco could be last since she has the fastest DPS with her shotgun.

I think Sachiho>Mirea>TGO>Usaco would be more optimal for future runs.

Sub hour should be possible with some corrections.