The run:

I believe that this run used a keyboard macro program (ex. AutoHotKey) or something similar, which would technically make it tool assisted. There are a lot of details, small and large, that seem to point to this. This post isn't enough to reject the run right away, since I'm far from an expert on how exactly it would have been pulled off, but hopefully this is enough to prove it should be looked into further.

My argument comes mainly from the symbol spamming in different forms, which is used almost everywhere and in every level. Almost none of it shows any real strategy, with one exception being the level 1 boss. Most of the time it is cycling through one of every move in a pattern, taking 3-5 frames for each, with some minor variations for special moves like lightning. Going frame by frame on these, especially the boss mashing on level 3, shows that the mouse is alternating between a few set points instead of moving naturally. My argument isn’t too specific and has at least some bias, since I believed this run was sus from the start and had a run in 4th place prior to it getting accepted, but I still believe there's little to no chance Bluely’s run wasn't done without the use of an outside tool.